The Effect of Different Doses of Solid Biogas Wastes on Total Phenol Content and Antioxidant Activity of Wheat (Triticum aestivum L.)

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This study was conducted on the research and experimental fields at Adnan Menderes University Agriculture Faculty Research Farm during wheat growing period in 2016/2017. The experiment included the effects of three different mineral nitrogen fertilizer doses (0, 9, 18 kg/da) and five different solid biogas waste doses (0, 2, 4, 6, 8 t/da) and their combinations on the total phenol content and antioxidant activity of wheat. Total phenol content was determined by using gallic acid standard according to Folin-Ciocalteu method of grains of Ceyhan-99 variety. Determination of total antioxidant activity was performed using 2,2 diphenyl 1 picryl hydrazyl (DPPH) free radical. It is desirable that total phenolic content in bread wheat grain is high in order to prevent the damage of free radicals. When the results obtained in the study were examined, solid biogas waste application and mineral nitrogen application and their combinations caused total phenol content in bread wheat plant to change between 158.15-361.84 μg GAE/g. The highest phenolic content was obtained from the application of 18 kg N/da and 2 t/da solid biogas waste with 331.99 μg GAE/g, while the lowest total phenolic substance content was obtained from 215.83 μg 0 kg N/da and 2 t/da solid biogas waste application. Antioxidant activity varied between 16.17-29.68% and the highest antioxidant activity was obtained from 18 kg N/da and 2 t/da solid biogas waste application, while the lowest antioxidant activity was 9 kg N/da and 8 t/da also obtained in solid biogas waste application. In general, better results were obtained in combination with 2 t/da solid biogas waste and 18 kg/da mineral nitrogen dose.

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Wheat , biogase , antioxidant activity , total phenol content

Journal Of The Faculty Of Agriculture, Suleyman Demirel University

94

Journal Of The Faculty Of Agriculture, Suleyman Demirel University