Automated Fluorescence and Reflectance Coregistered 3-D Tissue Imaging System

Author

Shen, Zhaolong ; Nacev, Alek ; Sarwar, Azeem ; Lee, Roger ; Depireux, Didier ; Shapiro, Benjamin

Author_Institution

Fischell Dept. of Bioeng., Univ. of Maryland, College Park, MD, USA

Volume

49

Issue

1

fYear

2013

fDate

Jan. 2013

Firstpage

279

Lastpage

284

Abstract

An automated system was developed to image the 3-D distribution of fluorescent magnetic nanoparticles in tissue samples. It enables easy measurement of magnetic nanoparticle distributions, for small and large tissue samples (currently up to a maximum size of 25 mm × 25 mm × 25 mm), in 3-D, with about 70 resolution in plane and 1 μm in the vertical direction. There is a linear correlation between particle concentration and fluorescence intensity, hence the system provides a quantitative measure of the nanoparticle distribution, but the tissue sample is destroyed during the imaging process. The system was demonstrated by measuring the particle distribution in rat ear and brain samples.

Keywords

biological tissues; biomagnetism; biomedical measurement; biomedical optical imaging; brain; fluorescence; image registration; magnetic particles; medical image processing; nanobiotechnology; nanomagnetics; nanoparticles; reflectivity; automated fluorescence; brain samples; fluorescence intensity; fluorescent magnetic nanoparticles; magnetic nanoparticle distribution measurement; rat ear; reflectance coregistered 3D tissue imaging system; size 1 mum; Cameras; Ear; Magnetic cores; Magnetic resonance imaging; Nanoparticles; Optical imaging; Cryostat; drug targeting; fluorescence imaging; histology; image registration; magnetic nanoparticles; reflectance imaging; tissue imaging;

fLanguage

English

Journal_Title

Magnetics, IEEE Transactions on

Publisher

ieee

ISSN

0018-9464

Type

jour

DOI

10.1109/TMAG.2012.2222360

Filename

6392418