Title :
Automated Fluorescence and Reflectance Coregistered 3-D Tissue Imaging System
Author :
Shen, Zhaolong ; Nacev, Alek ; Sarwar, Azeem ; Lee, Roger ; Depireux, Didier ; Shapiro, Benjamin
Author_Institution :
Fischell Dept. of Bioeng., Univ. of Maryland, College Park, MD, USA
Abstract :
An automated system was developed to image the 3-D distribution of fluorescent magnetic nanoparticles in tissue samples. It enables easy measurement of magnetic nanoparticle distributions, for small and large tissue samples (currently up to a maximum size of 25 mm × 25 mm × 25 mm), in 3-D, with about 70 resolution in plane and 1 μm in the vertical direction. There is a linear correlation between particle concentration and fluorescence intensity, hence the system provides a quantitative measure of the nanoparticle distribution, but the tissue sample is destroyed during the imaging process. The system was demonstrated by measuring the particle distribution in rat ear and brain samples.
Keywords :
biological tissues; biomagnetism; biomedical measurement; biomedical optical imaging; brain; fluorescence; image registration; magnetic particles; medical image processing; nanobiotechnology; nanomagnetics; nanoparticles; reflectivity; automated fluorescence; brain samples; fluorescence intensity; fluorescent magnetic nanoparticles; magnetic nanoparticle distribution measurement; rat ear; reflectance coregistered 3D tissue imaging system; size 1 mum; Cameras; Ear; Magnetic cores; Magnetic resonance imaging; Nanoparticles; Optical imaging; Cryostat; drug targeting; fluorescence imaging; histology; image registration; magnetic nanoparticles; reflectance imaging; tissue imaging;
Journal_Title :
Magnetics, IEEE Transactions on
DOI :
10.1109/TMAG.2012.2222360
