Title :
Magnetic Barcode Nanowires for Osteosarcoma Cell Control, Detection and Separation
Author :
Sharma, Anirudh ; Zhu, Yuechen ; Thor, ShengShee ; Zhou, Fang ; Stadler, Bethanie ; Hubel, Allison
Author_Institution :
Electr. & Comput. Eng. Dept., Univ. of Minnesota, Minneapolis, MN, USA
Abstract :
Multilayered gold/nickel “barcode” nanowires were grown using electrodeposition for multiplexed detection in biomolecular assays. Non-specific uptake of barcoded nanowires by osteosarcoma cells (OSCA-8) was observed by scanning and transmission electron microscopy images. Barcode-tagged cells were manipulated with an external magnetic field and were readily separated from untagged cells. Separation efficiency increased from a maximum of 30.14% to a maximum of 47.9% as clustering was reduced. Cells exposed to nanowires (both those that incorporated wires and those that did not) appeared to be viable suggesting that the nanowires are not toxic.
Keywords :
biomagnetism; bone; cancer; cellular biophysics; electrodeposition; gold; magnetic multilayers; nanobiotechnology; nanofabrication; nanomagnetics; nanowires; nickel; scanning electron microscopy; toxicology; transmission electron microscopy; Au-Ni; biomolecular assay multiplexed detection; electrodeposition; multilayered gold-nickel barcode nanowires; osteosarcoma cell control; osteosarcoma cell detection; osteosarcoma cell separation; scanning electron microscopy images; toxicity; transmission electron microscopy image; Gold; Magnetic force microscopy; Magnetic resonance imaging; Magnetic separation; Microscopy; Nanowires; Nickel; Cell manipulation; cellular uptake; magnetic carriers; magnetic separation; nanowires;
Journal_Title :
Magnetics, IEEE Transactions on
DOI :
10.1109/TMAG.2012.2224647
