Title :
Microelectrode Array (MEA) Platform for Targeted Neuronal Transfection and Recording
Author :
Jain, Tilak ; Muthuswamy, Jit
Abstract :
Techniques used for nonviral gene transfection often have poor spatial resolution. In this letter, we present a microelectrode array (MEA) system that can precisely transfect exogenous molecules into targeted primary neurons using microelectroporation. An optimal cathodic pulse 4 V in amplitude and 1 ms in duration resulted in a transfection efficiency of 56% and a viability of 82%. Finally, siRNA molecules were transfected into targeted neurons in culture using the aforementioned system.
Keywords :
biomedical electrodes; genetics; microelectrodes; molecular biophysics; neurophysiology; cathodic pulse; microelectrode array platform; microelectroporation; nonviral gene transfection; siRNA molecules; targeted neuronal recording; targeted neuronal transfection; transfection efficiency; Biomedical engineering; Circuits; DNA; Fabrication; Interference; Microelectrodes; Neurons; Neuroscience; RNA; Spatial resolution; Biochip; electroporation; siRNA; transfection; Animals; Cell Culture Techniques; Electroporation; Gene Therapy; Microelectrodes; Neurons; Protein Array Analysis; RNA, Small Interfering; Rats; Rats, Sprague-Dawley; Transfection;
Journal_Title :
Biomedical Engineering, IEEE Transactions on
DOI :
10.1109/TBME.2007.914403
