Determination of Trace 1-Hydroxypyrene by Resin MN202 With Graphene Composite Modified Glassy Carbon Electrode

1-hydroxypyrene (1-OHP) is a kind of polycyclic aromatic hydrocarbons (PAHs) metabolites and has been used widely as a biomarker for evaluating human exposure to PAHs. In this paper, an electrochemical sensor was fabricated based on graphene-MN202 modified glassy carbon electrodes and used to preconcentrate and detect 1-OHP. When 1-OHP was preconcentrated on modified electrodes, the response signals and adsorption stability were enhanced. The electrochemical characteristics of 1-OHP on the modified electrode were investigated by cyclic voltammetry and differential pulse voltammetry. Experimental parameters were optimized, such as the adsorption potential, adsorption time, scan rate, and the pH values of buffer solution. Under the optimized conditions, the peak current was proportional to 1-OHP concentration in a wide range from 0.005 to 12.0 μm ol/L, and the detection limit was 1.72 nmol/L (S/N = 3). Moreover, the fabricated electrode also exhibited good reproducibility and stability, and can be employed to determinate 1-OHP, which is in human urine in situ successfully.