The arrival of genetic engineering

Author

Arulmuthu, Eugene R. ; Williams, David J. ; Versteeg, Hendrik K.

Author_Institution

Gene Therapy Consortium, UCL, London

Volume

28

Issue

1

fYear

2009

Firstpage

40

Lastpage

54

Abstract

The purpose of this article is to review the different strategies for the delivery of plasmid DNA (pDNA)-based gene therapy. Plasmids are extrachromosomal DNA capable of being transmitted from cell to cell. They are supercoiled, circular covalently closed (ccc) strands of DNA capable of replication independently of host DNA and ranging from 5 to 400 kilo base pairs (kb) in size, depending on the size of the therapeutic gene insert.

Keywords

DNA; cancer; cellular biophysics; diseases; gene therapy; genetic engineering; genetics; lung; molecular biophysics; aerosol gene delivery; chronic respiratory diseases; cystic fibrosis; extrachromosomal DNA; genetic engineering; influenza; lung cancer; nonviral gene therapeutics; plasmid DNA-based gene therapy; Bioinformatics; Clinical trials; DNA; Diseases; Gene therapy; Genetic engineering; Genomics; Humans; Lungs; Medical treatment; Cystic Fibrosis; Drug Administration Routes; Gene Therapy; Gene Transfer Techniques; Humans; Magnetics; Metered Dose Inhalers; Nebulizers and Vaporizers; Peptide Nucleic Acids; Plasmids;

fLanguage

English

Journal_Title

Engineering in Medicine and Biology Magazine, IEEE

Publisher

ieee

ISSN

0739-5175

Type

jour

DOI

10.1109/MEMB.2008.931015

Filename

4747722