Microbubble attenuation and destruction: are they involved in sonoporation efficiency?

Author

Escoffre, J. ; Novell, A. ; Piron, J. ; Zeghimi, A. ; Doinikov, A. ; Bouakaz, A.

Author_Institution

UMR Imagerie et Cerveau, Univ. Francois Rabelais, Tours, France

Volume

60

Issue

1

fYear

2013

fDate

Jan-13

Abstract

This technical note investigates the involvement of microbubble attenuation and destruction in sonoporation mechanisms. First, we evaluate sonoporation efficiency using Vevo Micromarker, and a comparison is made with BR14 and SonoVue microbubbles. Then, the acoustical properties of the microbubbles are measured to gain insight into the sonoporation mechanisms using a green fluorescent protein as a marker. Using glioblastoma cells, an unprecedented transfection rate of 70% is reached with Vevo Micromarker, corresponding to a 1.5-fold increase compared with the rate achieved with the other microbubbles. Moreover, attenuation and destruction were shown to be two key parameters in sonoporation efficiency.

Keywords

biological effects of acoustic radiation; biomedical ultrasonics; biomembranes; bubbles; cellular biophysics; drug delivery systems; ultrasonic absorption; BR14 microbubbles; GFP marker; SonoVue microbubbles; Vevo Micromarker; glioblastoma cells; green fluorescent protein; microbubble acoustical properties; microbubble attenuation; microbubble destruction; sonoporation efficiency; Acoustic measurements; Acoustics; Attenuation; Attenuation measurement; Transducers; Ultrasonic imaging; Ultrasonic variables measurement; Cell Line, Tumor; Contrast Media; Genes, Reporter; Glioblastoma; Green Fluorescent Proteins; Humans; Microbubbles; Sonication; Time Factors; Transfection; Ultrasonics;

fLanguage

English

Journal_Title

Ultrasonics, Ferroelectrics, and Frequency Control, IEEE Transactions on

Publisher

ieee

ISSN

0885-3010

Type

jour

DOI

10.1109/TUFFC.2013.2536

Filename

6396485