Title :
A sensitive polarized fluorometer for clinical tests
Author :
Lago, Paolo ; Rovati, Luigi ; Corti, Mario
Author_Institution :
Dept. of Electron., Pavia Univ., Italy
fDate :
8/1/1994 12:00:00 AM
Abstract :
A fully automated fluorometer which measures fluorescence depolarization is described. The instrument has been designed to work with standard microtiter sample holders normally used in clinical tests. The combination of a hybrid optical system, built with discrete optical elements and fiber bundles, and a digital Fourier analysis of the detected signals make the instrument quite sensitive and precise. The 96 samples are scanned in about 100 s, with a reproducibility of the measurement of the polarization ratio of about 10-3 and a minimum content of fluorescein in the sample well of 20 pmol
Keywords :
Fourier analysis; biomedical equipment; biomedical measurement; computerised spectroscopy; light polarisation; medical signal processing; optical fibres; automated fluorometer; clinical tests; computer control; digital Fourier analysis; discrete optical elements; fiber bundles; fluorescein; fluorescence depolarization; hybrid optical system; microtiter sample holders; polarization ratio; reproducibility; sensitive polarized fluorometer; signal analysis; Batteries; Buttocks; Instruments; Microcomputers; Orthotics; Polarization; Surface treatment; Testing; Transducers; Wire;
Journal_Title :
Instrumentation and Measurement, IEEE Transactions on
