Quantifying the effect of interconnections on the steady states of biomolecular networks

Interconnections of biomolecular networks result in new steady states, compared to isolated subsystems, due to the context-dependent effect of perturbations of subsystem “local” parameters like total enzyme availabilities. This paper presents a new theoretical approach, and an algorithm derived from the approach, which allows the determination of the signs of global changes in steady states upon perturbation of parameters in individual subsystems.