Rapid Specific and Label-Free Detection of Porcine Rotavirus Using Photonic Crystal Biosensors

Author

Pineda, Maria Fernanda ; Chan, Leo Li-Ying ; Kuhlenschmidt, Theresa ; Choi, Charles J. ; Kuhlenschmidt, Mark ; Cunningham, Brian T.

Author_Institution

Dept. of Bioeng., Univ. of Illinois at Urbana-Champaign, Urbana, IL

Volume

9

Issue

4

fYear

2009

fDate

4/1/2009 12:00:00 AM

Firstpage

470

Lastpage

477

Abstract

A rapid, label-free assay using photonic crystal biosensors for the direct detection of intact viable rotaviruses is demonstrated. This paper describes the design and operation of the biosensor, surface functionalization with anti-rotavirus antibody, characterization of the detection sensitivity, and demonstration of specificity with respect to non-target virus particles. A 30-min assay of a partially processed water sample yielded a detection sensitivity of 36 virus focus forming units without the use of any external reagents. This sensitivity is comparable to commercial enzyme-linked immunosorbent assays. These results suggest photonic crystal biosensors may be useful for real-time monitoring of virus contamination of environmental water resources.

Keywords

biological techniques; biosensors; contamination; health and safety; microorganisms; optical sensors; photonic crystals; water resources; antirotavirus antibody; environmental water resources; ground water monitoring; intact viable rotavirus detection; label-free detection; photonic crystal biosensors; porcine rotavirus; surface functionalization; time 30 min; virus contamination; Acoustic signal detection; Biomedical optical imaging; Biosensors; Immune system; Monitoring; Optical sensors; Pathogens; Photonic crystals; Water pollution; Water resources; Ground water monitoring; label-free detection; photonic crystal biosensor; rotavirus;

fLanguage

English

Journal_Title

Sensors Journal, IEEE

Publisher

ieee

ISSN

1530-437X

Type

jour

DOI

10.1109/JSEN.2009.2014427

Filename

4797920