Milligauss magnetic field applied pure water exert firefly luciferin-luciferase luminescence and induce intracellular calcium elevation of CHO cells without ATP

In the previous paper, we demonstrated that milligauss ultra-low-frequency alternative current (mG ULF-AC) magnetic field applied phosphate buffered saline solution (PBS) per se stimulated elevation of intracellular Ca²⁺ level and enhanced phagocytic activity of human poly-morphonuclear (PMN) leukocyte. During the course of the study to explore the mechanism of the biological effect of such mG magnetic field applied PBS, we found extraordinary phenomena which challenges our understanding of physical chemistry of water and bioenergetics. PBS and highly purified water were exposed to mG ULF-AC which was generated by a Helmholtz coil under visible light (mG/light). When mG/light-applied pure water or PBS was added to the medium, CHO cells which respond to exogenous adenosine triphosphate (ATP) resulting in increase of intracellular Ca²⁺ level, were found to elevate intracellular Ca²⁺ level without exogenous ATP. This activity of mG ULF-AC applied water or PBS was equivalent to 100 nM ATP. Furthermore, when magnetic field was applied on highly purified water under visible light, for more than 12 h at 40°C, the pure water became to exert luminescence of luciferin in the presence of luciferase. This reaction did not require ATP nor Mg²⁺ in the reaction mixture at all. These facts indicate that mG/light-applied PBS or water mimics ATP activity.