Light Scattering Study on Motile Spermatozoa

The light scattering technique with a photoelectron counting method was applied to determine the speed distribution of spermatozoa, when a short measurement time and a weak illuminating light are prerequisite to avoid the effects of deterioration and light injury. The result is quantitatively in good agreement with the one obtained in other methods. It was found that the theory developed from the light scattering study of bacteria in artificial solution can be applied without modification to the light scattered from motile spermatozoa in semen containing various adulterations. Therefore, this technique proves to be useful in finding the accurate motility of spermatozoa in a very short observation time.