DocumentCode
1224119
Title
Recovery of very low intensity CCD images from noise
Author
Kolbe, W.F. ; Turko, B.T.
Author_Institution
Lawrence Berkeley Lab., CA, USA
Volume
36
Issue
1
fYear
1989
fDate
2/1/1989 12:00:00 AM
Firstpage
731
Lastpage
733
Abstract
A scheme for detecting low-intensity images with a CCD (charge coupled device) camera is described in which successive images are digitized at fast frame rates and averaged to enhance the signal-to-noise ratio. The results demonstrate that a dynamic range in excess of 10000:1 can be obtained using an uncooled device and averaging times of 100 s, a sensitivity comparable to that obtained with cooled devices. The technique is illustrated by imaging the weak fluorescence produced by an ethidium bromide stained DNA electrophoresis gel
Keywords
DNA; charge-coupled devices; electrophoresis; fluorescence; gels; photoluminescence; radiation detection and measurement; CCD camera; DNA electrophoresis gel; averaging times; ethidium bromide stained; fluorescence; low intensity CCD images; noise; signal-to-noise ratio; Charge coupled devices; Charge-coupled image sensors; Computer displays; Dark current; Detectors; Light sources; Noise generators; Noise reduction; Signal to noise ratio; Temperature;
fLanguage
English
Journal_Title
Nuclear Science, IEEE Transactions on
Publisher
ieee
ISSN
0018-9499
Type
jour
DOI
10.1109/23.34537
Filename
34537
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