• DocumentCode
    1225739
  • Title

    Application of the Electrical Sizing Principle of Coulter to a New Multiparameter System

  • Author

    Groves, Michael R.

  • Author_Institution
    Coulter Electronics, Inc.
  • Issue
    7
  • fYear
    1980
  • fDate
    7/1/1980 12:00:00 AM
  • Firstpage
    364
  • Lastpage
    369
  • Abstract
    A new technique is described for simultaneously measuring, within 100 ¿s, cell volume, membrane breakdown voltage, and internal conductivity on individual cells of microscopic dimensions. The system is a modification of the hydrodynamically focusing Coulter Counter®. Tumor cells established from rat fetal brain cells of average volume 2100 ¿m3 were measured. A breakdown voltage of 0.86 V was found with an apparent volume underestimation after breakdown of 40 percent.
  • Keywords
    Biomembranes; Breakdown voltage; Cells (biology); Conductivity; Counting circuits; Dielectric breakdown; Electric breakdown; Fluorescence; Orifices; Volume measurement; Animals; Cell Membrane; Electric Conductivity; Oncogenic Viruses; Rats;
  • fLanguage
    English
  • Journal_Title
    Biomedical Engineering, IEEE Transactions on
  • Publisher
    ieee
  • ISSN
    0018-9294
  • Type

    jour

  • DOI
    10.1109/TBME.1980.326649
  • Filename
    4123276
    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=49&DC=1225739