Title :
Compact semiconductor light-emitting diodes for dynamic imaging of neuronal circuitry
Author :
Venkataramani, Sowmya ; Davitt, Kristina M. ; Zhang, Jiayi ; Xu, Heng ; Song, Yoon-Kyu ; Connors, Barry W. ; Nurmikko, Arto V.
Author_Institution :
Dept. of Phys., Brown Univ., Providence, RI, USA
Abstract :
We describe the use of compact green light-emitting diodes (LEDs) for fluorescence optical imaging and demonstrate this for dynamic recording from cultured neurons. The efficient gallium-nitride-based LEDs have individual element sizes comparable to typical biological cells and are operated in proximity illumination mode for individual neurons. As periodic arrays, and with direct electrical control of each LED, a spatially periodic multicellular target can be induced to fluoresce in a predesigned spatiotemporal sequence, thereby providing a new approach to dynamic recording of small neural circuits.
Keywords :
III-V semiconductors; bioelectric phenomena; biomedical equipment; biomedical optical imaging; cellular biophysics; fluorescence; gallium compounds; light emitting diodes; neurophysiology; semiconductor devices; spatiotemporal phenomena; GaN; biological cells; compact green LED; cultured neurons; direct electrical control; dynamic imaging; dynamic recording; fluorescence optical imaging; gallium-nitride-based LED; illumination mode; neural circuits; neuronal circuitry; semiconductor light-emitting diodes; spatially periodic multicellular target; spatiotemporal sequence; Biological cells; Circuits; Fluorescence; Gallium; III-V semiconductor materials; Light emitting diodes; Lighting; Neurons; Optical imaging; Optical recording; Light-emitting diodes (LEDs); optical imaging; patterned hippocampal neurons; voltage-sensitive dye (VSD) imaging;
Journal_Title :
Selected Topics in Quantum Electronics, IEEE Journal of
DOI :
10.1109/JSTQE.2005.857722
