Measuring cellular structure at submicrometer scale with light scattering spectroscopy

Author

Backman, Vadim ; Gopal, Venkatesh ; Kalashnikov, Maxim ; Badizadegan, Kamran ; Gurjar, Rajan ; Wax, Adam ; Georgakoudi, Irene ; Mueller, Markus ; Boone, Charles W. ; Dasari, Ramachandra R. ; Feld, Michael S.

Author_Institution

Dept. of Biomed. Eng., Northwestern Univ., Evanston, IL, USA

Volume

7

Issue

6

fYear

2001

Firstpage

887

Lastpage

893

Abstract

We present a novel instrument for imaging the angular distributions of light backscattered by biological cells and tissues. The intensities in different regions of the image are due to scatterers of different sizes. We exploit this to study scattering from particles smaller than the wavelength of light used, even when they are mixed with larger particles. We show that the scattering from subcellular structure in both normal and cancerous human cells is best fitted to inverse power-law distributions for the sizes of the scattering objects, and propose that the distribution of scattering objects may be different in normal versus cancerous cells

Keywords

bio-optics; biomedical imaging; cancer; cellular biophysics; fractals; light scattering; particle size measurement; refractive index measurement; tumours; angular distributions; backscattered light; biological cells; biological tissues; cancerous human cells; cellular structure; chromatin; fractal; imaging instrument; inverse power-law distributions; light scattering spectroscopy; normal human cells; normal mesothelial cells; polystyrene microspheres; refractive index; spectral distributions; subcellular structure; tumor colon cells; Biological tissues; Cancer; Humans; Light scattering; Optical refraction; Optical scattering; Particle scattering; Proteins; Solids; Spectroscopy;

fLanguage

English

Journal_Title

Selected Topics in Quantum Electronics, IEEE Journal of

Publisher

ieee

ISSN

1077-260X

Type

jour

DOI

10.1109/2944.983289

Filename

983289