Fast and precise positioning of single cells on planar electrode substrates

Author

Thielecke, Hagen ; Stieglitz, Thomas ; Beutel, Hansjoerg ; Matthies, Till ; Ruf, Hans Heinrice ; Meyer, Jorg-Uwe

Author_Institution

Dept. of Microsyst., Fraunhofer Inst. for Biomed. Eng., Germany

Volume

18

Issue

6

fYear

1999

Firstpage

48

Lastpage

52

Abstract

For cell biosensors and for studying neural networks using planar electrode substrates, a suitable technique for positioning single cells on electrodes was needed. We reported a new method for fast and efficient positioning of single cells on ring electrodes by controlled suction through holes. We described the microfabrication of electrode substrates with microholes and the cell positioning procedure. L929 cells and Neuro 2A cells could be positioned in parallel without cell damage.

Keywords

adhesion; biocontrol; biological specimen preparation; biomedical electrodes; biosensors; cellular biophysics; micropositioning; microsensors; neurophysiology; sedimentation; L929 cells; Neuro 2A cells; cell adhesion; cell biosensors; controlled suction through holes; fast precise positioning; hydrodynamic force; microfabrication; microholes; micromachining; neural networks; planar electrode substrates; ring electrodes; sedimentation; single cell positioning; Adhesives; Electrodes; Engineering drawings; Etching; Hydrodynamics; Large Hadron Collider; Lithography; Pressure control; Silicon; Substrates; Aluminum; Animals; Biosensing Techniques; Cell Adhesion; Cell Count; Cell Division; Cell Line; Ceramics; Equipment Design; Fibroblasts; Gold; Mice; Microelectrodes; Neural Networks (Computer); Neuroblastoma; Semiconductors; Silicon Compounds; Suction; Surface Properties; Tumor Cells, Cultured;

fLanguage

English

Journal_Title

Engineering in Medicine and Biology Magazine, IEEE

Publisher

ieee

ISSN

0739-5175

Type

jour

DOI

10.1109/51.805144

Filename

805144