Title :
Near-Common-Path Self-Reference Quantitative Phase Microscopy
Author :
Hillman, Timothy R. ; Lue, Niyom ; Sung, Yongjin ; Dasari, Ramachandra R. ; Yaqoob, Zahid
Author_Institution :
Laser Biomed. Res. Center, Massachusetts Inst. of Technol., Cambridge, MA, USA
Abstract :
We report a near-common-path self-reference quantitative phase microscope, wherein a quantitative phase image is formed through the off-axis interference of the sample wave with a 180-degree rotated version of itself. A pair of dove prisms, oriented in different directions, is used to effect the relative transformation between the beams. Our technique features a simple optical design that requires no maintenance, rendering it accessible to nonspecialists in the field of optics. Additionally, its variable magnification and low-noise phase measurement capabilities make it suitable for high-accuracy imaging of biological samples of different sizes.
Keywords :
biological techniques; light interference; optical design techniques; optical images; optical microscopy; optical prisms; phase measurement; biological samples; dove prisms; high-accuracy imaging; low-noise phase measurement capability; near-common-path self-reference quantitative phase microscopy; off-axis interference; optical design; quantitative phase image; sample wave; Image reconstruction; Microscopy; Optical interferometry; Optical microscopy; Optimized production technology; Phase measurement; Bioinstrumentation; interferometry; optical imaging; phase retrieval;
Journal_Title :
Photonics Technology Letters, IEEE
DOI :
10.1109/LPT.2012.2214768
