Title :
Ultrasensitive analysis with the crossed-beam thermal lens
Author :
Nolan, Thomas G. ; Dovichi, Norman J.
Author_Institution :
Dept. of Chem., Wyoming Univ., Laramie, WY, USA
Abstract :
The crossed-beam thermal lens is a two-beam laser technique that produces a high-spatial resolution measurement of weak optical absorbance. The technique has been used for scanning microscope, nonintrusive flow measurements, and liquid chromatographic detection. The authors report the detection of 120 analyte molecules within a 2×10-10 cm3 volume of liquid. The possibility of detecting and counting single antibodies is considered.
Keywords :
biological techniques and instruments; measurement by laser beam; spectrochemical analysis; thermo-optical effects; analyte molecules; antibody counting; crossed-beam thermal lens; high-spatial resolution measurement; liquid chromatographic detection; molecular detection; nonintrusive flow; photothermal refraction; scanning microscope; two-beam laser technique; ultrasensitive analysis; weak optical absorbance; Chemical lasers; Laser beams; Laser excitation; Lenses; Measurement by laser beam; Pump lasers; Thermal lensing;
Journal_Title :
Circuits and Devices Magazine, IEEE
DOI :
10.1109/MCD.1986.6311772
