Title :
Aligned microcontact printing of micrometer-scale poly-L-Lysine structures for controlled growth of cultured neurons on planar microelectrode arrays
Author :
James, C.D. ; Davis, R. ; Meyer, M. ; Turner, A. ; Turner, S. ; Withers, G. ; Kam, L. ; Banker, G. ; Craighead, H. ; Issacson, M. ; Turner, J. ; Shain, W.
Author_Institution :
Sch. of Appl. & Eng. Phys., Cornell Univ., Ithaca, NY, USA
Abstract :
We describe a method for producing high-resolution chemical patterns on surfaces to control the attachment and growth of cultured neurons. Microcontact printing has been extended to allow the printing of μm-scale protein lines aligned to an underlying pattern of planar microelectrodes. Poly-L-lysine (PL) lines have been printed on the electrode array for electrical studies on cultured neural networks. Rat hippocampal neurons showed degree of attachment selectivity to the PL and produced neurites that faithfully grew onto the electrode recording sites.
Keywords :
biological specimen preparation; biomedical electrodes; cellular biophysics; microelectrodes; neurophysiology; photolithography; proteins; aligned microcontact printing; controlled growth; cultured neurons; degree of attachment selectivity; electrode recording sites; geometrical control; high-resolution chemical patterns; micrometer-scale poly-L-Lysine structures; multisite recording; neurites; photolithography; planar microelectrode arrays; profiled master; protein lines; rat hippocampal neurons; thin stamp; Biomedical engineering; Chemicals; Electrodes; Extracellular; Microelectrodes; Neurons; Physics; Proteins; Soft lithography; Surface topography; Animals; Cells, Cultured; Dimethylpolysiloxanes; Equipment Design; Extracellular Matrix; Hippocampus; Microelectrodes; Neural Networks (Computer); Neurons; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Silicones; Surface Properties; Transistors;
Journal_Title :
Biomedical Engineering, IEEE Transactions on
