Development of a Microwave—Accelerated Metal-Enhanced Fluorescence 40 Second, <100 cfu/mL Point of Care Assay for the Detection of Chlamydia Trachomatis

Author

Zhang, Yongxia ; Agreda, Patricia ; Kelley, Shannon ; Gaydos, Charlotte ; Geddes, Chris D.

Author_Institution

Dept. of Chem. & Biochem., Univ. of Maryland Baltimore County, Baltimore, MD, USA

Volume

58

Issue

3

fYear

2011

fDate

3/1/2011 12:00:00 AM

Firstpage

781

Lastpage

784

Abstract

An inexpensive technology to both lyse Chlamydia trachomatis (CT) and detect DNA released from CT within 40 s is demonstrated. In a microwave cavity, energy is highly focused using 100-nm gold films with “bow-tie” structures to lyse CT within 10 s. The ultrafast detection of the released DNA from less than 100 cfu/mL CT is accomplished in an additional 30 s by employing the microwave-accelerated metal-enhanced fluorescence technique. This new “ release and detect” platform technology is a highly attractive alternative method for the lysing of bacteria, DNA extraction, and the fast quantification of bacteria and potentially other pathogenic species and cells as well. Our approach is a significant step forward for the development of a point of care test for CT.

Keywords

DNA; biochemistry; biological effects of microwaves; biological techniques; biomolecular effects of radiation; cellular effects of radiation; fluorescence; microorganisms; microwave spectra; molecular biophysics; spectrochemical analysis; DNA detection; DNA extraction; assay platform; bacteria lysing; chlamydia trachomatis; focused microwave burst; gold films; microwave cavity; microwave-accelerated metal-enhanced fluorescence; morphological changes; pathogenic species; size 100 nm; time 40 s; Acceleration; DNA; Diseases; Electromagnetic heating; Fluorescence; Gold; Microorganisms; Microwave technology; Microwave theory and techniques; Testing; Chlamydia trachomatis (CT); lysis; microwave-accelerated metal-enhanced fluorescence (MAMEF); Base Sequence; Chlamydia trachomatis; DNA, Bacterial; Microscopy, Electron, Transmission; Microwaves; Molecular Diagnostic Techniques; Molecular Sequence Data; Nucleic Acid Hybridization; Point-of-Care Systems; Spectrometry, Fluorescence;

fLanguage

English

Journal_Title

Biomedical Engineering, IEEE Transactions on

Publisher

ieee

ISSN

0018-9294

Type

jour

DOI

10.1109/TBME.2010.2066275

Filename

5546921