Title :
Photobleaching of Cy5 Conjugated Lipid Bilayers Determined With Optical Microresonators
Author :
Freeman, L.M. ; Armani, Andrea M.
Author_Institution :
Dept. of Nanoengineering, Univ. of California San Diego, San Diego, CA, USA
Abstract :
Whispering gallery-mode optical microcavities have significantly impacted the field of label-free optical biodetection. By combining the evanescent field generated by the microcavity with biomimetic surface chemistries, it is possible to use the microcavities to study the fundamental photobleaching behavior of fluorescent molecules embedded in biomaterials. In this study, a self-assembled Cy5-conjugated lipid bilayer is formed on a spherical optical microcavity. The evanescent tail of the microsphere is used to excite the Cy5 dye molecules. Both the emission wavelength and the fluorescence intensity decrease of the Cy5-conjugated dye in the lipid bilayer are measured.
Keywords :
DNA; RNA; biochemistry; biomembranes; biomimetics; dyes; enzymes; fluorescence; lipid bilayers; microcavities; micromechanical resonators; molecular biophysics; optical resonators; optical saturable absorption; self-assembly; surface chemistry; whispering gallery modes; Cy5 dye molecules; biomimetic surface chemistry; emission wavelength; evanescent field; fluorescence intensity; fluorescent molecules; label-free optical biodetection; optical microresonators; photobleaching behavior; self-assembled Cy5-conjugated lipid bilayer; spherical optical microcavity; whispering gallery-mode optical microcavities; Cavity resonators; Fluorescence; Lipidomics; Microcavities; Optical fibers; Optical surface waves; Fluorescent dyes; lipid bilayers; photobleaching; whispering gallery-mode resonators;
Journal_Title :
Selected Topics in Quantum Electronics, IEEE Journal of
DOI :
10.1109/JSTQE.2011.2166059
