DocumentCode
133759
Title
An implantable window system for chronic two-photon microscopy on mouse organs
Author
Komai, Yutaka
Author_Institution
WPI Immunology Frontier Res. Center, Osaka Univ., Osaka, Japan
fYear
2014
fDate
3-7 Aug. 2014
Firstpage
406
Lastpage
410
Abstract
Animal models were developed for chronic observation with using two-photon microscopy. To demonstrate potency of the models, T-cell and macrophage migration in inguinal lymph node, spleen, abdominal cavity and kidney were observed for two weeks on single mice. The image quality was maintained high enough during an observation period to count and trace cell population and migration.
Keywords
biomedical optical imaging; cell motility; kidney; medical image processing; optical microscopy; two-photon processes; T-cell migration; abdominal cavity; chronic two-photon microscopy; image quality; implantable window system; inguinal lymph node; kidney; macrophage migration; mouse organs; spleen; time 2 week; Biomedical imaging; Fluorescence; Green products; Laser excitation; Laser modes; Mice; Surgery; T cell; chronic observation; homeostatic proliferation; two-photon microscopy;
fLanguage
English
Publisher
ieee
Conference_Titel
World Automation Congress (WAC), 2014
Conference_Location
Waikoloa, HI
Type
conf
DOI
10.1109/WAC.2014.6935973
Filename
6935973
Link To Document