Title :
Use of silicon and GaAs pixel detectors for digital autoradiography
Author :
Amendolia, S.R. ; Beccherle, R. ; Bertolucci, E. ; Bisogni, M.G. ; Bottigli, U. ; Campbell, M. ; Chesi, E. ; Ciocci, M.A. ; Conti, M. ; Via, C. Da ; Guerra, A. Del ; D´Auria, S. ; Fantacci, M.E. ; Gambaccini, M. ; Grossi, G. ; Heijen, E. ; Mancini, E. ; M
Author_Institution :
Istituto di Fisica, Sassari Univ., Italy
fDate :
6/1/1997 12:00:00 AM
Abstract :
Solid state detectors made of Si (4.8/spl times/8 mm/sup 2/) and GaAs (6.4/spl times/8 mm/sup 2/) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelial cells and DNA fragments separated via electrophoresis, both labeled with /sup 32/P. The system has shown a good minimum detectable activity per unit area of 2.10/sup -4/ cps mm/sup -2/, and has proved linear for a count rate in the range 0.2-20 cpa, typical of autoradiography. The pixel dimensions are 75/spl times/500 /spl mu/m/sup 2/ (Si-Omega2) and 50/spl times/500 /spl mu/m/sup 2/ (GaAs-Omega3), respectively. We are able to clearly localize clusters of cells which have incorporated the radioactive tracer and DNA fragments on an electrophoretic gel on paper (blots).
Keywords :
DNA; biological techniques; cellular biophysics; radioactive tracers; radioisotope imaging; semiconductor counters; silicon radiation detectors; /sup 32/P; 4.8 mm; 50 mm; 500 mm; 6.4 mm; 75 mm; 8 mm; DNA fragments; GaAs; GaAs pixel detectors; P; Si; Si pixel detectors; digital autoradiography; electrophoretic gel; human epithelial cells; minimum detectable activity per unit area; paper; pixel dimensions; Biomedical imaging; DNA; Detectors; Gallium arsenide; Medical diagnostic imaging; Phosphors; Pixel; Silicon; Solid state circuits; Spatial resolution;
Journal_Title :
Nuclear Science, IEEE Transactions on
