Development of chemical stimulation system for local environment control by using combination of spout and suction from dual-pipettes

Single cell analysis has attracted much attention for revealing detailed and localized biological information. A local environmental control technique is desired when analyzing the detailed and localized properties of single cells. In this paper, we propose a local environmental stimulation system with micro dual-pipettes to stimulate the local reagent concentration dynamically, freely and automatically. When the local environment is controlled with chemical stimuli, the chemical solution is diffused and affects other objects. The local environment stimulation system with micro dual-pipettes can solve this problem. By using spout pipette and suction pipette, it is possible to use local stimuli without affecting other objects. For evaluating the locality of the system, we use a fluorescent substance and measure the diffusion of the spouted solution.