In vitro simultaneous measurement of refractive index and thickness of biological tissue by the low coherence interferometry

Author

Ohmi, Masato ; Ohnishi, Yasuhito ; Yoden, Koji ; Haruna, Masamitsu

Author_Institution

Fac. of Med., Osaka Univ., Japan

Volume

47

Issue

9

fYear

2000

Firstpage

1266

Lastpage

1270

Abstract

The authors proposed and demonstrated in vitro simultaneous measurement of refractive index and thickness of biological tissue. The technique is based on the low coherence interferometry combined with precise translation stages. Refractive indices were determined with the accuracy of less than 1% for tissue samples of a few hundred micron thickness, including chicken tissue, human tooth and nail. Simultaneous measurement of refractive index and thickness of multilayer tissue are also demonstrated.

Keywords

bio-optics; biological techniques; biological tissues; light coherence; light interferometry; refractive index measurement; thickness measurement; biological research technique; chicken tissue; human nail; human tooth; low coherence interferometry; multilayer tissue; precise translation stages; Biological tissues; Biomedical measurements; Biomedical optical imaging; Humans; In vitro; Optical interferometry; Optical refraction; Optical variables control; Refractive index; Thickness measurement; Animals; Biomedical Engineering; Chickens; Humans; Interferometry; Nails; Optics; Refractometry; Scattering, Radiation; Tooth;

fLanguage

English

Journal_Title

Biomedical Engineering, IEEE Transactions on

Publisher

ieee

ISSN

0018-9294

Type

jour

DOI

10.1109/10.867961

Filename

867961