Title :
XPS and NEXAFS studies of VUV/O3-treated aromatic polyurea and its application to microchip electrophoresis
Author :
Shinohara, Hirofumi ; Nakahara, Akihito ; Kitagawa, Fumio ; Takahashi, Y. ; Otsuka, Kanji ; Shoji, Shuji ; Ohara, O. ; Mizuno, Jun
Author_Institution :
Fac. of Sci. & Eng., Waseda Univ., Tokyo, Japan
fDate :
12/1/2011 12:00:00 AM
Abstract :
In this study, the authors performed X-ray photoelectron spectroscopy (XPS) and near-edge X-ray absorption fine structure (NEXAFS) studies of vacuum ultraviolet (VUV)/O3-treated aromatic polyurea films to investigate their treatment effects. XPS and NEXAFS spectra indicate that the benzene ring was cleaved after treatment and that carboxyl, hydroxyl, ketone and aldehyde groups were formed at the cleaved sites. The VUV/O3-treated polyurea film was applied to a polymethylmethacrylate (PMMA) microchip for microchip electrophoresis (MCE) of bovine serum albumin (BSA). Fast electro-osmotic mobility of 4.6 × 10-4 cm2/V/s as well as reduction of the BSA adhesion was achieved. This functional surface is useful for high-speed MCE analysis.
Keywords :
EXAFS; X-ray photoelectron spectra; XANES; bioelectric phenomena; biological techniques; electrophoresis; molecular biophysics; osmosis; polymer films; proteins; ultraviolet radiation effects; BSA adhesion; NEXAFS; VUV-O3-treated aromatic polyurea; X-ray photoelectron spectroscopy; XPS; aldehyde group; benzene ring; bovine serum albumin; carboxyl group; cleaved sites; electro-osmotic mobility; high-speed MCE analysis; hydroxyl group; ketone group; microchip electrophoresis; near-edge X-ray absorption fine structure; polymethylmethacrylate microchip; vacuum ultraviolet-treated aromatic polyurea films;
Journal_Title :
Nanobiotechnology, IET
DOI :
10.1049/iet-nbt.2011.0006
