Title :
Microelectrode arrays for electrophysiological monitoring of hippocampal organotypic slice cultures
Author :
Thiébaud, P. ; de Rooij, N.F. ; Koudelka-Hep, M. ; Stoppini, L.
Author_Institution :
Inst. of Microtechnology, Neuchatel Univ., Switzerland
Abstract :
A three-dimensional platinum (Pt) microelectrode array embedded on a micromachined silicon (Si) substrate (porosity of 13%, via hole diameter of 40 μm) has been developed. Electrodes are 35-μm wide and 20-μm high, spaced 200 μm apart and arranged in an elliptic geometry. Integrated within a microperfusion chamber, the devices were used for stimulation and recording experiments of hippocampal slice cultures over a period of several days.
Keywords :
arrays; bioelectric potentials; biological techniques; brain; laboratory techniques; microelectrodes; monitoring; 20 mum; 200 mum; 35 mum; 40 mum; Pt; Si; electrophysiological monitoring; elliptic geometry; embedded three-dimensional platinum microelectrode array; hippocampal organotypic slice cultures; hole diameter; micromachined silicon substrate; microperfusion chamber; neuroscience method; porosity; recording experiments; stimulation experiments; Biomembranes; Electrodes; Etching; Fabrication; In vitro; Microelectrodes; Monitoring; Passivation; Plasma applications; Silicon; Animals; Electric Conductivity; Electrophysiology; Equipment Design; Evoked Potentials; Hippocampus; Microelectrodes; Monitoring, Physiologic; Organ Culture Techniques; Platinum; Rats; Rats, Wistar;
Journal_Title :
Biomedical Engineering, IEEE Transactions on
