Nanograting structure promotes lamellipodia-based cell collective migration and wound healing

Author

Zaozao Chen ; Atchison, Leigh ; HaYeun Ji ; Leong, Kam W.

Author_Institution

Dept. of Biomed. Eng., Duke Univ., Durham, NC, USA

fYear

2014

fDate

26-30 Aug. 2014

Firstpage

2916

Lastpage

2919

Abstract

Wound healing is a dynamic and complex process of replacing missing or dead cell structures and tissue layers. The aim of this research is to discover biocompatible materials and drugs that can promote cell migration in the wound area and thus enhance desirable wound healing effects. In this paper, we report that PDMS nanogratings could accelerate the migration of epithelial cells along the grating axis, and the addition of Imatinib could further increase the epithelial cell wound healing speed to 1.6 times the speed of control cells. We also demonstrate that this migration is mediated by lamellipodia protrusion, and is Rac1-GTPase activity dependent. Lastly, we discuss the potential application and prospect of different nanostructured biomaterials for wound healing studies.

Keywords

biomedical materials; cell motility; drugs; enzymes; molecular biophysics; nanomedicine; nanostructured materials; polymers; tissue engineering; wounds; Imatinib; PDMS nanogratings; Rac1-GTPase activity; biocompatible materials; drugs; epithelial cell migration; epithelial cell wound healing speed; grating axis; lamellipodia protrusion; lamellipodia-based cell collective migration; nanograting structure; nanostructured biomaterials; tissue layers; wound healing; Adhesives; Inhibitors; Nanostructures; PD control; Substrates; Wounds;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society (EMBC), 2014 36th Annual International Conference of the IEEE

Conference_Location

Chicago, IL

ISSN

1557-170X

Type

conf

DOI

10.1109/EMBC.2014.6944233

Filename

6944233