Title :
Robust estimation for class averaging in Cryo-EM Single Particle Reconstruction
Author :
Chenxi Huang ; Tagare, Hemant D.
Author_Institution :
Dept. of Biomed. Eng., Yale Univ., New Haven, CT, USA
Abstract :
Single Particle Reconstruction (SPR) for Cryogenic Electron Microscopy (cryo-EM) aligns and averages the images extracted from micrographs to improve the Signal-to-Noise ratio (SNR). Outliers compromise the fidelity of the averaging. We propose a robust cross-correlation-like w-estimator for combating the effect of outliers on the average images in cryo-EM. The estimator accounts for the natural variation of signal contrast among the images and eliminates the need for a threshold for outlier rejection. We show that the influence function of our estimator is asymptotically bounded. Evaluations of the estimator on simulated and real cryo-EM images show good performance in the presence of outliers.
Keywords :
cryogenics; electron microscopy; estimation theory; image denoising; image reconstruction; medical image processing; cryo-EM imaging; cryo-EM single particle reconstruction; cryogenic electron microscopy aligns; image extraction; micrographs; robust cross-correlation-like w-estimator; robust estimation; signal contrast; signal-noise ratio; Correlation; Image reconstruction; Microscopy; Robustness; Signal to noise ratio; Three-dimensional displays;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2014 36th Annual International Conference of the IEEE
Conference_Location :
Chicago, IL
DOI :
10.1109/EMBC.2014.6944335