Title :
Cytotoxicity of synthesized Iron Oxide nanoparticles: Toward novel biomarkers of colon cancer
Author :
Raji, M. Ahmadzadeh ; Amara, M. ; Amoabediny, G. ; Tajik, P. ; Barin, A. ; Magierowski, S. ; Ghafar-Zadeh, Ebrahim
Author_Institution :
Dept. of Nano-Biotechnol., Univ. of Tehran, Tehran, Iran
Abstract :
In this paper we present the preliminary results of a novel biological analysis platform for early colon cancer detection using magnetic separation of magnetized markers. The platform consists of a microfluidic structure integrated with biosensors. Super-Paramagnetic Iron Oxide nanoparticles (SPIO-NPs) were functionalized with purified DNA Aptamer and their synthesis is described. In this paper, we also present the physicochemical results of the synthesized SPIO/Au-NPs characterized by TEM and XRD. Toxicity of our synthesized biomarkers on HCT116 cell line is discussed. Based on our findings, a concentration of 1mg/ml of our biomarkers added to 5 × 105 cells per well has no effect the viability of the human cells even after 24 hours.
Keywords :
DNA; X-ray diffraction; bioMEMS; biosensors; cancer; cellular biophysics; chemical sensors; gold; iron compounds; magnetic particles; microfluidics; nanomedicine; nanoparticles; patient diagnosis; superparamagnetism; toxicology; transmission electron microscopy; Au; HCT116 cell line; SPIO-NP; TEM; XRD; biological analysis platform; biomarker concentration; biosensors; early colon cancer detection; human cell viability; iron oxide nanoparticle cytotoxicity; magnetic separation; magnetized markers; microfluidic structure; physicochemical results; purified DNA Aptamer; superparamagnetic iron oxide nanoparticles; Cancer; Colon; Iron; Magnetic field measurement; Magnetic resonance imaging; Magnetic separation; Nanoparticles;
Conference_Titel :
Engineering in Medicine and Biology Society (EMBC), 2014 36th Annual International Conference of the IEEE
Conference_Location :
Chicago, IL
DOI :
10.1109/EMBC.2014.6945040
