DocumentCode :
1433042
Title :
N-carboxyethylchitosan/nanohydroxyapatite composites scaffold for tracheal cartilage tissue-engineering applications
Author :
Hong-can Shi ; Dan Lu ; Wan-Peng Wang ; Yan Lu ; Yan-Jun Zeng
Author_Institution :
Coll. of Clinical Med., Yangzhou Univ., Yangzhou, China
Volume :
7
Issue :
1
fYear :
2012
fDate :
1/1/2012 12:00:00 AM
Firstpage :
76
Lastpage :
79
Abstract :
The aim of this Letter is to investigate the feasibility of isolated tracheal chondrocytes on a novel-type nanocomposite scaffold for application to cartilage regeneration. The scaffold was based on biocompatible N-carboxyethylchitosan containing hydroxyapatite nanoparticles (NCECS/nHA). NCECS/nHA nanocomposite scaffold was fabricated by vacuum freeze-drying. Tracheal cartilage segments from a 8-week-old New Zealand white rabbit were chopped into small 1 × 1 × 1 × mm3 blocks. Following type II collagenase digestion, chondrocytes at passage 3 were seeded onto three-dimensional porous NCECS/nHA scaffolds. These cell-scaffold constructs were precultured in the incubator for 5 days and then subcutaneously implanted into nude mice for in vivo culture. After 8 weeks, explanted constructs were harvested and dedicated for histomorphologic and biochemical assays. The pore size of NCECS/nHA nanocomposite scaffold ranged from 200 to 500 m. The porosity was 85 . The chondrocytes cultured in this scaffold produced cartilaginous matrices. Immunohistochemical staining for type II collagen revealed that chondrocytes exhibited dark brown cytoplasm. After 8-week in vivo culture, the neonatal cartilage-like tissue exhibited histomorphological and biochemical characteristics similar to that of mature hyaline cartilage. These results demonstrated that NCECS/nHA facilitated the adhesion and viability of chondrocytes, and therefore allowed the authors to make further improvement by applying tissue-engineering methods to regenerate tracheas for clinical use.
Keywords :
adhesion; biochemistry; biomedical materials; calcium compounds; cellular biophysics; enzymes; molecular biophysics; nanocomposites; nanomedicine; polymers; porosity; tissue engineering; N-carboxyethylchitosan; New Zealand white rabbit; adhesion; biochemical assay; biocompatible N-carboxyethylchitosan; cartilage regeneration; cartilaginous matrix; cell-scaffold construct; chondrocyte; composite scaffold; dark brown cytoplasm; histomorphologic assay; hydroxyapatite nanoparticle; immunohistochemical staining; incubator; isolated tracheal chondrocyte; mature hyaline cartilage; nanocomposite scaffold; nanohydroxyapatite; neonatal cartilage-like tissue; three-dimensional porous NCECS-nHA scaffold; time 8 wk; tracheal cartilage tissue-engineering application; type II collagen; type II collagenase digestion; vacuum freeze-drying;
fLanguage :
English
Journal_Title :
Micro & Nano Letters, IET
Publisher :
iet
ISSN :
1750-0443
Type :
jour
DOI :
10.1049/mnl.2011.0628
Filename :
6140926
Link To Document :
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