Optical Tweezer Technology

Author

Tan, Youhua ; Leung, Anskar Y H ; Wang, Kaiqun ; Fung, Tsz-Kan ; Sun, Dong

Author_Institution

City Univ. of Hong Kong, Kowloon, China

Volume

5

Issue

1

fYear

2011

fDate

3/1/2011 12:00:00 AM

Firstpage

17

Lastpage

21

Abstract

The optical tweezer, with the ability to apply force and deformation on a micro scaled object on the order of piconewton (pN, 10~12 N) and nanometer (nm, 10~9 m), is utilized in this study to manipulate primitive myeloblasts from acute myeloid leukemia (AML) patients for biomechanical properties characterization. Mcrobeads are attached to cell surfaces serving as handles. Cells are stretched by moving the beads with optical traps and progressively increasing the distance between them. Through force calibration and image processing, the relationship between stretching force and induced cell deformation can be established, from which the biomechanical properties of AML myeloblasts are characterized based on a computational model. The findings in this study will provide insights into the cell mechanics of primitive AML hematopoietic cells.

Keywords

biological techniques; biomechanics; cellular biophysics; diseases; radiation pressure; AML myeloblast biomechanical properties; AML patients; acute myeloid leukemia; biomechanical properties characterization; cell surfaces; computational model; force calibration; image processing; induced cell deformation; microbeads; optical traps; optical tweezer technology; primitive AML hematopoietic cell mechanics; primitive myeloblast manipulation; stretching force; Biomedical equipment; Blood; Bone tissue; Cancer; Chemotherapy; Medical services; Optical materials;

fLanguage

English

Journal_Title

Nanotechnology Magazine, IEEE

Publisher

ieee

ISSN

1932-4510

Type

jour

DOI

10.1109/MNANO.2010.939832

Filename

5716837