A CMOS/Thin-Film Fluorescence Contact Imaging Microsystem for DNA Analysis

Author

Singh, Ritu Raj ; Ho, Derek ; Nilchi, Alireza ; Gulak, Glenn ; Yau, Patrick ; Genov, Roman

Author_Institution

Dept. of Electr. & Comput. Eng., Univ. of Toronto, Toronto, ON, Canada

Volume

57

Issue

5

fYear

2010

fDate

5/1/2010 12:00:00 AM

Firstpage

1029

Lastpage

1038

Abstract

A hybrid CMOS/thin-film microsystem for fluorescence contact imaging is presented. The microsystem integrates a high-performance optical interference filter and a 128 ?? 128 pixel active pixel sensor fabricated in a standard 0.35-??m CMOS technology. The thin-film filter has an optical density greater than 6.0 at the wavelength of interest, providing adequate excitation rejection to the 532-nm solid-state laser. Microsystem performance is experimentally validated by imaging spots of Cyanine-3 fluorophore, conventionally used in DNA detection. The emission intensity as a function of fluorophore concentration is measured with an estimated sensitivity of 5000 fluorophore/??m² . A human DNA microarray has been imaged with the sensor prototype.

Keywords

CMOS image sensors; DNA; biomedical optical imaging; interference filters; lab-on-a-chip; micromechanical devices; CMOS-thin film microsystem; Cyanine-3 fluorophore; DNA analysis; DNA microarray; fluorescence contact imaging; optical density; optical filter; optical interference filter; Contact imaging; DNA detection; fluorescence imaging; microarray; optical filter;

fLanguage

English

Journal_Title

Circuits and Systems I: Regular Papers, IEEE Transactions on

Publisher

ieee

ISSN

1549-8328

Type

jour

DOI

10.1109/TCSI.2010.2043990

Filename

5439822