Hyperpolarization of Human Mesenchymal Stem Cells in Response to Magnetic Force

Author

Kirkham, Glen R. ; Elliot, Kerry J. ; Keramane, Ahmed ; Salter, Donald M. ; Dobson, Jon P. ; El Haj, Alicia J. ; Cartmell, Sarah Harriet

Author_Institution

Inst. of Sci. & Technol. in Med., Univ. of Keele, Stoke-on-Trent, UK

Volume

9

Issue

1

fYear

2010

fDate

3/1/2010 12:00:00 AM

Firstpage

71

Lastpage

74

Abstract

Magnetic particle tagging techniques are currently being applied to tissue engineering applications such as controlled differentiation of mesenchymal stem cells (MSC). In order to define key mechanotransducers underpinning these applications, the electrophysiological responses of human MSCs (hMSC) have been investigated. Ferromagnetic microparticles were coated with L-arginyl-glycyl-L-aspartic acid in order to target the application of dynamic force (6 pN) directly to cell surface integrins. Human MSCs demonstrated cell membrane hyperpolarization responses after the application of force, mediated by BK channels and intracellular calcium release.

Keywords

bioelectric phenomena; biomembrane transport; cellular biophysics; magnetic particles; tissue engineering; BK channels; L-arginyl-glycyl-L-aspartic acid; cell differentiation; cell membrane hyperpolarization responses; cell surface integrins; dynamic force; electrophysiological responses; ferromagnetic microparticles; human mesenchymal stem cells; intracellular calcium release; magnetic force; magnetic particle tagging; mechanotransducers; tissue engineering; BK channels; calcium channel; electrophysiology; magnetic particles; mechanotransduction; mesenchymal stem cells (MSCs); Analysis of Variance; Calcium Channels; Cell Polarity; Cells, Cultured; Electromagnetic Fields; Electrophysiology; Ferric Compounds; Humans; Large-Conductance Calcium-Activated Potassium Channels; Membrane Potentials; Mesenchymal Stem Cells; Nifedipine; Thapsigargin;

fLanguage

English

Journal_Title

NanoBioscience, IEEE Transactions on

Publisher

ieee

ISSN

1536-1241

Type

jour

DOI

10.1109/TNB.2010.2042065

Filename

5440016