DocumentCode :
1527543
Title :
Quantitative analyses of changes in intracellular calcium ion concentrations under flow-induced mechanical stimuli utilising a microchip
Author :
So Hee Park ; Ji Won Shin ; Dong-Hwa Kim ; Su-Hyang Kim ; Ok Chan Jeong ; Jeong Koo Kim ; Jung-Woog Shin
Author_Institution :
Dept. of Biomed. Eng., Inje Univ., Gimhae, South Korea
Volume :
6
Issue :
5
fYear :
2011
fDate :
5/1/2011 12:00:00 AM
Firstpage :
296
Lastpage :
300
Abstract :
The authors use quantitative engineering methods to investigate changes in intracellular calcium ion concentrations ([Ca2+]i) under various flow-induced shear stresses utilising a microchip. MG-63 cells were seeded onto microchannels in a chip made of polydimethylsiloxane. A commercially available gear pump was utilised to induce and control the shear stress for up to 20 min. The temperature was maintained at 35±1°C using a computer-controlled heating jacket. Five types of flow patterns, each of which consisted of equal stimulation and rest intervals (50, 100, 150 and 200 s for stimulation, rest and steady flow) were tested. Each interval produced different patterns indicating the [Ca2+]i. Various engineering parameters were extracted from the [Ca2+]i signals recorded from a single cell after filtering to reduce background noise, including the peak to peak interval, trends in the changes of the peak values based on an exponential function and the ratio of stimulation energy to [Ca2+]i. The results indicate that this protocol can be used to analyse [Ca2+]i and related cellular responses to shearing stresses.
Keywords :
biological fluid dynamics; biomembrane transport; calcium; cellular biophysics; chemical analysis; lab-on-a-chip; microchannel flow; positive ions; MG-63 cells; background noise; computer-controlled heating jacket; flow patterns; flow-induced mechanical stimuli; flow-induced shear stresses; gear pump; intracellular calcium ion concentrations; microchannels; microchip; polydimethylsiloxane; quantitative analyses; quantitative engineering methods;
fLanguage :
English
Journal_Title :
Micro & Nano Letters, IET
Publisher :
iet
ISSN :
1750-0443
Type :
jour
DOI :
10.1049/mnl.2011.0018
Filename :
5775897
Link To Document :
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