The use of derivatized magnetoliposomes for extraction of antibodies from aqueous solutions

Author

Dumitrascu, Gabriela ; Kumbhar, Amar ; Zhou, Weilie ; Rosenzweig, Zeev

Author_Institution

Dept. of Chem., New Orleans Univ., LA, USA

Volume

37

Issue

4

fYear

2001

fDate

7/1/2001 12:00:00 AM

Firstpage

2932

Lastpage

2934

Abstract

The paper describes the synthesis of magnetoliposomes derivatized with fluorescent ligands and their use for the detection and extraction of antibodies from aqueous solutions. The magnetoliposomes contain cobalt platinum alloy nanoparticles that were annealed prior to encapsulation in the liposomes. Transmission electron microscopy (TEM) images and SQUID magnetometry measurements show that the annealing process improves their room temperature magnetic properties. To demonstrate their extraction power, magnetoliposomes labeled with BODIPY-Fluorescein were used to extract antibodies against BODIPY-Fluorescein from aqueous solution

Keywords

annealing; biological techniques; biomagnetism; cobalt alloys; fluorescence; magnetic particles; nanostructured materials; platinum alloys; solutions; transmission electron microscopy; BODIPY-Fluorescein label; Co-Pt; SQUID magnetometry; annealing; antibody detection; antibody extraction; aqueous solution; bioassay; cobalt platinum alloy nanoparticles; derivatized magnetoliposome synthesis; encapsulation; fluorescent ligand; magnetic properties; transmission electron microscopy; Annealing; Cobalt; Encapsulation; Fluorescence; Magnetic force microscopy; Nanoparticles; Platinum alloys; SQUIDs; Temperature; Transmission electron microscopy;

fLanguage

English

Journal_Title

Magnetics, IEEE Transactions on

Publisher

ieee

ISSN

0018-9464

Type

jour

DOI

10.1109/20.951351

Filename

951351