Title :
Two-photon excitation imaging based on a compact scanning head
Author :
Diaspro, A. ; Corosu, Mirko ; Ramoino, Paola ; Robello, Mauro
Author_Institution :
Dept. of Phys., Genoa Univ., Italy
Abstract :
The adaptation of a confocal laser scanning microscope to a two-photon architecture for 3-D fluorescence microscopy is described.
Keywords :
bio-optics; biological techniques; biomedical equipment; biomedical imaging; fluorescence; optical microscopes; optical microscopy; two-photon processes; 3D fluorescence microscopy; compact scanning head; confocal laser scanning microscope; optical sectioning effect; point spread function; reduced scattering effects; two-photon architecture; two-photon excitation imaging; Absorption; Fluorescence; Head; Laser excitation; Microscopy; Optical imaging; Optical pulses; Physics; Spectroscopy; Ultrafast optics; Animals; Microscopy, Confocal; Microscopy, Fluorescence; Models, Theoretical; Paramecium;
Journal_Title :
Engineering in Medicine and Biology Magazine, IEEE
