Title :
Innovations in two-photon deep tissue microscopy
Author :
Buehler, Christof ; Kim, Ki Hean ; Dong, Chen Y. ; Masters, Barry R. ; So, Peter T.C.
Author_Institution :
Dept. of Mech. Eng., MIT, Cambridge, MA, USA
Abstract :
The goal of this article is to provide a review of deep tissue studies based on two-photon microscopy. We will further explore three new developments that have significantly enhanced the power of two-photon imaging: (1) simultaneous two-photon fluorescence and confocal reflected-light imaging, (2) two-photon video-rate imaging, and (3) fluorescent spectroscopic measurements in deep tissue.
Keywords :
bio-optics; biological tissues; biomedical imaging; fluorescence; fluorescence spectroscopy; optical microscopy; reviews; two-photon processes; two-photon spectroscopy; biomedical applications; confocal reflected-light imaging; fluorescent spectroscopic measurements; simultaneous imaging; tissue models; two-photon deep tissue microscopy; two-photon fluorescence imaging; two-photon imaging; two-photon video-rate imaging; Anatomy; Biological system modeling; Cornea; Dermis; Fluorescence; Microscopy; Physiology; Skin; Spectroscopy; Technological innovation; Animals; Cornea; Humans; Microscopy, Confocal; Microscopy, Fluorescence; Microscopy, Video; Rabbits; Skin; Skin Physiology;
Journal_Title :
Engineering in Medicine and Biology Magazine, IEEE
