Culturing primary mouse embryonic fibroblasts in a microfluidic system

Primary mouse embryonic fibroblasts (mEFs) were cultured in a microfluidic system for a period of 8 days. The perfusion flow rate for these cells is different from those used for 3T3 fibroblasts and human adult fibroblasts due to the strong dependence on cell secreted factors. Primary mouse embryonic fibroblasts have not been cultured in a microfluidic system before. Our results have potential applications for these cells reprogrammed to a pluripotent state and important implications for primary cell culture using microdevices.