Title :
Culturing primary mouse embryonic fibroblasts in a microfluidic system
Author :
Villa, M.M. ; Pope, S.E. ; Conover, J.C. ; Fan, T.-H.
Author_Institution :
Dept. of Mech. Eng., Univ. of Connecticut, Storrs, CT
Abstract :
Primary mouse embryonic fibroblasts (mEFs) were cultured in a microfluidic system for a period of 8 days. The perfusion flow rate for these cells is different from those used for 3T3 fibroblasts and human adult fibroblasts due to the strong dependence on cell secreted factors. Primary mouse embryonic fibroblasts have not been cultured in a microfluidic system before. Our results have potential applications for these cells reprogrammed to a pluripotent state and important implications for primary cell culture using microdevices.
Keywords :
bioMEMS; biorheology; cellular biophysics; microfluidics; 3T3 fibroblasts; cell culturing; cell secretion factors; human adult fibroblasts; microdevices; microfluidic system; perfusion flow rate; pluripotent state; primary mouse embryonic fibroblasts; time 8 day; Cells (biology); Chemical analysis; Embryo; Fibroblasts; Glass; Medical control systems; Mice; Microfluidics; Plasma devices; Silicon;
Conference_Titel :
Bioengineering Conference, 2009 IEEE 35th Annual Northeast
Conference_Location :
Boston, MA
Print_ISBN :
978-1-4244-4362-8
Electronic_ISBN :
978-1-4244-4364-2
DOI :
10.1109/NEBC.2009.4967648
