Title :
SCN1A point mutations on neuron connectivity
Author :
Krishnamurthy, K. ; Smith-Parker, A. ; Burton, S.D. ; Zeringue, H.C.
Author_Institution :
Dept. of Bioeng., Univ. of Pittsburgh, Pittsburgh, PA
Abstract :
Action potential generation and propagation is a critical parameter in the function of a neuronal network. The SCN1A voltage-gated sodium channel is an axonal protein crucial to action potential functionality. Here we have demonstrated the expression of a series of SCN1A genes in HEK 293 and primary neuronal cultures. The genes consist of a wildtype SCN1A as well as two point-mutation sequences. Expression was demonstrated using both immunocytochemistry and western blotting.
Keywords :
bioelectric potentials; cellular biophysics; genetics; molecular biophysics; neurophysiology; proteins; HEK 293 cell; SCN1A gene; SCN1A point mutation; SCN1A voltage-gated sodium channel; Western blotting; action potential generation; action potential propagation; axonal protein; immunocytochemistry; neuron connectivity; neuronal culture; neuronal network; point-mutation sequence; Biological neural networks; Biomembranes; Calcium; Epilepsy; Fluorescence; Genetic mutations; Immune system; Neurons; Proteins; Voltage;
Conference_Titel :
Bioengineering Conference, 2009 IEEE 35th Annual Northeast
Conference_Location :
Boston, MA
Print_ISBN :
978-1-4244-4362-8
Electronic_ISBN :
978-1-4244-4364-2
DOI :
10.1109/NEBC.2009.4967722
