Chondrogenic differentiation of adult mesenchymal stem cells and embryonic stem cells

Author

Shu, K. ; Thatte, H. ; Spector, M.

Author_Institution

Massachusetts Inst. of Technol., Cambridge, MA

fYear

2009

Firstpage

1

Lastpage

2

Abstract

Mesenchymal stem cell (MSC) contraction associated with chondrogenesis is attributed to the expression of alpha-smooth muscle actin (alpha-SMA). In this study, pluripotent embryonic carcinoma cells (ECCs) and MSCs were compared for cartilage histogensis. Both cell types expressed alpha-SMA in monolayer. However, when cultured in pellets and in 3-D scaffolds, only MSCs contracted and formed glycosaminoglycan (GAG)- and type II collagen-rich tissue. Under these culture conditions, MSCs appear to be superior over ECCs for cartilage regeneration.

Keywords

biological tissues; cellular biophysics; molecular biophysics; proteins; tissue engineering; 3D scaffolds; adult mesenchymal stem cells; alpha-smooth muscle actin; cartilage histogensis; cartilage regeneration; chondrogenesis; chondrogenic differentiation; embryonic stem cells; glycosaminoglycan; pluripotent embryonic carcinoma cells; type II collagen-rich tissue; Bones; Cells (biology); DNA; Electrochemical machining; Embryo; Immune system; Mice; Muscles; Proteins; Stem cells;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference, 2009 IEEE 35th Annual Northeast

Conference_Location

Boston, MA

Print_ISBN

978-1-4244-4362-8

Electronic_ISBN

978-1-4244-4364-2

Type

conf

DOI

10.1109/NEBC.2009.4967739

Filename

4967739