Lethal Effects of Steep Pulsed Electric Field (SPEF) to Target Lymphatic Capillaries in VX2Implanted Breast Cancer of Rabbits

This paper discussed the lethal effects of steep pulsed electric field (SPEF) to target lymphatic capillaries in VX₂ implanted breast cancer of rabbits using methylene blue dye injection staining, 5´-AMP-ALPase enzyme histochemical double staining, electron microscopy and VEGF-C observations. The result of methylene blue dye perfusion showed that the edge of tumor treated with SPEF was indistinct, no circularity or liner structure. Enzyme histochemical staining examination displayed that no positive staining was found in SPEF-target region of breast tumor, and lymphatic capillaries presented piecemeal structure in the edge of target region. Transmission electron microscopic examination showed that the continuity and integrality of lymphatic capillaries in tumor were destroyed after the treatment of SPEF. The expression of VEGF-C decreased markedly. The experiment indicated that SPEF induced the severe destruction of tumor cell and lymphatic capillaries in target region, and inhibited proliferation of lymphatic. Therefore it can decrease the possibility of lymphatic metastasis