Title :
Isolation, identification and characterization of four oil degrading strains
Author :
Wang, Ruijian ; Wang, Dongxue ; Shi, Yan ; Sun, Peng ; Yu, Xiaoguang ; Du, Fengguo
Author_Institution :
Provincial Key lab. of Forestry and Ecological Environment, Forestry College of Beihua University, Jilin 132013, China
Abstract :
Using crude oil as the sole carbon source, four indigenous microorganisms with oil degradation, JH250-5, JH250-8, JH250-10, and JH250-12, were isolated from heavy oil polluted soil. The oil degradation rate of JH250-8 was over 90% which was much higher than other strains. 16RNA gene sequencing results showed those four strains are all belong to Acinetobacter. GC and GC-MS analysis indicate that, the four strains mainly degrade the hydrocarbons of crude oil. Those strains have the potential application value in decontamination of oil pollution or alkane degrading reactions.
Keywords :
16s RNA gene sequencing; Acinetobacter; GC-MS; oil degradation;
Conference_Titel :
World Automation Congress (WAC), 2012
Conference_Location :
Puerto Vallarta, Mexico
Print_ISBN :
978-1-4673-4497-5