Disappearance of epileptic seizures on rat hippocampal slices using laser irradiation

The final purpose of our research is to cripple the epileptogenic focus by laser irradiation. However, the effect of laser irradiation on brain nerves is not well known. In this paper, we observed laser irradiation to CA3 region of rat hippocampal slices in the experiment. The rat hippocampal slices were kindled by electric stimulation for spontaneously generating epileptic seizures. A laser is a semiconductor pulse laser (the wavelength, the pulse width and the frequency are 808 nm, 990 μ sec and 50 Hz, respectively). The energy of the laser is variable between 6.08-313.3 mJ/cm². The laser irradiator is connected to an optical fiber whose diameter is 1mm. The experiment was delivered by the laser pulse, the energy of which over 30.4 mJ/cm². Epileptic seizures were disappeared by laser irradiation. The energy of laser light is higher, when the time of disappearing is shorter. After laser irradiation, electric responses of CAI and DG are still appeared. These results show that the laser irradiation give damaged to rat hippocampal slices and disappeared to epileptic seizures. We observed the epileptic seizures are disappeared by laser irradiation. We going to We going to research about laser parameters (the wavelength, the irradiation time), effective region by laser light, penetrance of laser light and all that.