Title :
A novel organophosphorus pesticides sensitive EN-FET
Author :
Yuan Zhong Yi ; Hong Mei ; Wlodarski, W.B. ; Ji Xing Song
Author_Institution :
Shanghai Inst. of Biochem., China
Abstract :
The authors describe a novel organophosphorus pesticide sensitive solid-state sensor, the enzyme modified field-effect transistor (EN-FET), for measurements in liquid media, based on the immobilized enzyme probe (IEP) technique using butyrylcholinesterase. The pH-EISFET with n-channel and silicon dioxide/silicon nitride insulator layers is used as the transducer. Butyrylcholinesterase has been covalently bound onto the gate of pH-ISFET (ion-sensitive FET) using glutaraldehyde and the bovine serum albumin. The organophosphorous pesticide sensitive EN-FET has the advantage of fast response.<>
Keywords :
biological techniques and instruments; biosensors; electric sensing devices; insulated gate field effect transistors; organic compounds; pH measurement; proteins; SiO/sub 2/-Si/sub 3/N/sub 4/; bovine serum albumin; butyrylcholinesterase; enzyme modified field-effect transistor; glutaraldehyde; human food chains; immobilisation; immobilized enzyme probe; ion-sensitive FET; organophosphorus pesticides; pH-ISFET; solid-state sensor; Amino acids; Biochemistry; Equations; FETs; Inhibitors;
Conference_Titel :
Solid-State Sensors and Actuators, 1991. Digest of Technical Papers, TRANSDUCERS '91., 1991 International Conference on
Conference_Location :
San Francisco, CA, USA
Print_ISBN :
0-87942-585-7
DOI :
10.1109/SENSOR.1991.148979
