Title :
Application of X-ray Synchrotron microscopy instrumentation in Biology
Author :
Gasperini, F.M. ; Pereira, G.R. ; Granjeiro, J.M. ; Calasans-Maia, M.D. ; Rossi, A.M. ; Perez, C.A. ; Lopes, R.T. ; Lima, I.
Author_Institution :
Med. Sci. Program, Fluminense Fed. Univ., Niteroi, Brazil
Abstract :
X-ray microfluorescence imaging technique has been used as a significant tool in order to investigate minerals contents in some kinds of materials. The aim of this study was to evaluate the elemental distribution of calcium and zinc in bone substitute materials (nanohydroxyapatite spheres) and cortical bones through X-Ray Microfluorescence analysis with the increment of Synchrotron Radiation in order to evaluate the characteristics of the newly formed bone and its interface, the preexisting bone and biomaterials by the arrangement of collagen fibers and its birefringence. The elemental mapping was carried out at Brazilian Synchrotron Light Laboratory, Campinas - Sao Paulo, Brazil working at D09-XRF beam line. Based on this study, the results suggest that hydroxyapatite-based biomaterials are biocompatible, promote osteoconduction and favored bone repair.
Keywords :
X-ray apparatus; X-ray fluorescence analysis; X-ray microscopy; bioceramics; birefringence; bone; calcium compounds; proteins; synchrotron radiation; Brazilian synchrotron light laboratory; Ca10(PO4)6(OH)2; Campinas; D09-XRF beam line; Sao Paulo; X-ray microfluorescence analysis; X-ray microfluorescence imaging technique; X-ray synchrotron microscopy instrumentation; biocompatibility analysis; biology; biomaterials; birefringence; bone characteristics; bone repair process; bone substitute materials; calcium elemental distribution; collagen fibers; cortical bones; elemental mapping method; hydroxyapatite-based biomaterials; mineral content analysis; nanohydroxyapatite spheres; synchrotron radiation; zinc elemental distribution; Bones; Calcium; Laboratories; Materials; Nanobioscience; X-ray imaging; Zinc;
Conference_Titel :
Advancements in Nuclear Instrumentation Measurement Methods and their Applications (ANIMMA), 2011 2nd International Conference on
Conference_Location :
Ghent
Print_ISBN :
978-1-4577-0925-8
DOI :
10.1109/ANIMMA.2011.6172972
