Title :
Microfermentors for the rapid screening and analysis of biochemical processes
Author :
Zanzotto, Andrea ; Szita, Nicolas ; Schmidt, Martin A. ; Jensen, Klavs F.
Author_Institution :
Dept. of Chem. Eng., MIT, Cambridge, MA, USA
fDate :
6/24/1905 12:00:00 AM
Abstract :
We report on a microfermentor fabricated from glass and poly(dimethylsiloxane) using techniques of soft lithography. Aeration of the bacterial culture is through a highly permeable membrane, and dissolved oxygen is monitored with the oxygen-sensitive, fluorescent compound Ru II tris(4,7-diphenyl-1,10-phenanthroline). Biomass within the microfermentor is monitored by measuring light transmission through the medium and relating this to the optical density. We present results on the fermentation of E. coli in this system
Keywords :
biochemistry; biosensors; biotechnology; fermentation; microfluidics; microorganisms; microsensors; optical sensors; rapid prototyping (industrial); bacterial culture aeration; batch fermentation; biochemical processes; biocompatibility; biomass; bioprocess science; bioreactors; dissolved oxygen; fluorescence quenching; light transmission; microfermentor; optical density; optical sensing; parallel fermentation; permeable membrane; photolithography; poly(dimethylsiloxane); rapid screening; soft lithography; sterilization; Biochemical analysis; Biomass; Biomedical optical imaging; Biomembranes; Density measurement; Fluorescence; Glass; Microorganisms; Monitoring; Soft lithography;
Conference_Titel :
Microtechnologies in Medicine & Biology 2nd Annual International IEEE-EMB Special Topic Conference on
Conference_Location :
Madison, WI
Print_ISBN :
0-7803-7480-0
DOI :
10.1109/MMB.2002.1002306
