Title :
Egr-1 expression induced by ZnO nanoparticles in human keratinocytes
Author :
Jeong, Sang Hoon ; Ryu, Hwa Jung ; Park, Yoon-Hee ; Bae, Hyun Cheol ; Son, Sang Wook
Author_Institution :
Dept. of Dermatology, Korea Univ., Seoul, South Korea
Abstract :
Zinc oxide (ZnO) nanoparticles (NPs) are widely used in cosmetics and sunscreen. In spite of the broad application of ZnO NPs on human skin, there are limited literatures on the potential toxicities of ZnO NPs at the cellular and molecular levels. The aim of this study was to investigate the signaling pathways of ZnO NPs-induced early growth response-1 (Egr-1) expression and the role of Egr-1 in ZnO NPs-induced cytokine expression. ZnO NPs increased the Egr-1 expression, promoter activity and its nuclear translocation in HaCaT cells. ZnO NPs activated extracellular signal-regulated kinase (ERK) of mitogen-activated protein kinase (MAPK) pathways. Up-regulation of Egr-1 expression by ZnO NPs stimulation was found to be inhibited by an ERK inhibitor, but by neither c-Jun-N-terminal kinase (JNK) nor p38 inhibitor. Our results showed that ZnO NPs induces Egr-1 expression via MAPK pathway in human keratinocytes and cytokine expression by Egr-1. These pathways may contribute to NPs-induced cutaneous toxicity.
Keywords :
biochemistry; cellular biophysics; cosmetics; enzymes; inhibitors; nanomedicine; nanoparticles; toxicology; zinc compounds; ERK inhibitor; Egr-1 expression; HaCaT cells; ZnO; ZnO NPs-induced early growth response-1; c-Jun-N-terminal kinase; cellular levels; cosmetics; cutaneous toxicity; cytokine expression; extracellular signal-regulated kinase; human keratinocytes; human skin; mitogen-activated protein kinase pathways; molecular levels; nuclear translocation; signaling pathways; sunscreen; toxicities; up-regulation; zinc oxide nanoparticles; Biomedical measurements; Humans; Proteins; USA Councils; Zinc oxide; Egr-1; Nanotoxicity; ZnO nanoparticls;
Conference_Titel :
Nanotechnology (IEEE-NANO), 2012 12th IEEE Conference on
Conference_Location :
Birmingham
Print_ISBN :
978-1-4673-2198-3
DOI :
10.1109/NANO.2012.6321932