Title :
Development of "IISA-ATP" system for in situ microbial activity assessment in deep-sea environment
Author :
Aoki, Yusuke ; Fukuba, Tatsuhiro ; Yamamoto, Takatoki ; Fujii, Teruo
Author_Institution :
Inst. of Ind. Sci., Tokyo Univ., Tokyo
Abstract :
In this study, "Integrated in situ analyzer-ATP (IISA-ATP)" which is to estimate microbial activities in deep-sea environments has been developed. On the system, a microfluidic device is used as a core element to perform a bioluminescent (luciferin-luciferase) reaction for ATP quantification. The microfluidic device has a coil-shaped microchannel for photo intensity measurement. In this paper, concept of the IISA-ATP and optimization process of the microchannel design are presented. As a result of the microchannel design and flow rate optimization, 2 pM to 2 x 104 pM (20 nM) of ATP was successfully quantified.
Keywords :
oceanographic equipment; IISA-ATP; Integrated in situ analyzer-ATP; bioluminescent reaction; deep-sea environments; flow rate optimization; luciferin-luciferase; microbial activities; microchannel design; microfluidic device; photo intensity measurement; Bioluminescence; Chemical analysis; Design optimization; Extracellular; Microchannel; Microfluidics; Microorganisms; Ocean temperature; Sea measurements; Spatial resolution;
Conference_Titel :
OCEANS 2008 - MTS/IEEE Kobe Techno-Ocean
Conference_Location :
Kobe
Print_ISBN :
978-1-4244-2125-1
Electronic_ISBN :
978-1-4244-2126-8
DOI :
10.1109/OCEANSKOBE.2008.4530991
